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Uric Acid

Overview of Test

Analysis of the enzyme Uricase and the Uricase-Uric Acid reaction byproduct, hydrogen peroxide, for the quantitative determination of Uric Acid in human serum.

Author

Patrick

Specimen Types

Serum

Test Number

2233

Expected Turn Around Time

24 hours

CPT Codes

84550

Special Instructions

None

Preferred Volume

2 mL

Minimum Volume

1 mL

Collection Container or Device

SST

Collection Instructions Attached

No collection instructions attached

Storage Details/ Stability Requirements

Temperature
Room Temp: (15-30 C)
Refrigeration: (2-8 C)
Time Period
24 Hours
3 Days

Transport Requirements

Refrigerated

Causes For Rejection

Grossly Hemolyzed, Frozen in Gel Tube, QNS, Not Centrifuged

Methodology

Hydrogen peroxide, a byproduct of the Uricase-Uric Acid reaction, is coupled with other enzymatic reactions to yield a colorimetric end product. This uses the coupling of 4-aminoantipyrine (4-AAP), 2-Hydroxy-3,5-Dichloro-benzenesulfonate (HDCBS), and hydrogen peroxide in the presence of peroxidase to yield a chromagen measured photometrically to determine the concentration of Uric Acid within a sample.

Collection Instructions If Written

Department

Blood

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1250 Stephenson Highway, STE 200, Troy, MI 48083
info@totallabsolutions.com
+1 (248) 800-6626
+1 (248) 800-6656
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