Test Panels

Calcium

Overview of Test

The concentration of calcium in plasma is determined by the balance between calcium absorption and excretion, and by exchanges between plasma, extracellular fluid and tissues including bone. High levels of calcium can indicate hyperthyroidism and hypercalcemia of malignancy. Low levels of calcium indicate hypoparathyroidism, vitamin D deficiency, hereditary vitamin D dependency, rickets types 1 and 2 and impaired intestinal absorption.

Author

Patrick

Specimen Types

Serum

Test Number

2209

Expected Turn Around Time

1 day

CPT Codes

82310

Special Instructions

N/A

Preferred Volume

2 mL

Minimum Volume

1mL

Collection Container or Device

Serum Separator Tube (SST)

Collection Instructions Attached

No collection instructions attached

Storage Details/ Stability Requirements

Temperature
Room Temp: (15-30°C)
Refrigeration: (2-8°C)
Time Period
8-24 hours
3 days

Transport Requirements

Refrigerated

Causes For Rejection

Grossly hemolyzed. Frozen in gel tube. Specimen quantity not sufficient. Not centrifuged.

Methodology

Beckman Coulter AU680 analyzer with Carolina Liquid Chemistries reagent. Quantitative determination of total determination of total calcium. Calcium reacts with arsenazo 3 in the buffered reagent to form a blue complex. The absorbance of the reagent is measured bichromatically at 650 nm and 700 nm immediately before and 21 seconds after sample addition. The change in absorbance is proportional to the calcium concentration of the sample.

Collection Instructions If Written

After draw, allow the specimen to clot by letting it sit at room temperature for approximately 30 minutes. Specimens should be centrifuged within 2 hours of collection. Centrifuge the specimen at 3,400 RPMs for 15 minutes to separate the red blood cells from the serum.

Department

Blood

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