Test Panels

B-Type Natriuretic Peptide (BNP)

Overview of Test

Quantitative measurement of B-type natriuretic peptide in plasma specimens using the anticoagulant EDTA. This test is intended to be used to evaluate congestive heart failure, severity of congestive heart failure, risk of patients with acute coronary syndromes, and the risk stratification of patients with heart failure

Author

Kathryn

Specimen Types

Plasma

Test Number

5722

Expected Turn Around Time

1 Day

CPT Codes

83880

Special Instructions

For longer storage, please centrifuge the lavender-top EDTA tube and transfer at least 500 μL of cell free plasma into an aliquot tube. Clearly mark aliquot specimen as a lavender pour-off for BNP testing. Keep centrifuged EDTA tube and aliquot together. Freeze aliquot and transport frozen.

Preferred Volume

1 mL

Minimum Volume

0.5 mL

Collection Container or Device

Lavender-top EDTA tube

Collection Instructions Attached

No collection instructions attached

Storage Details/ Stability Requirements

Temperature
Room Temp: (15-30° C)
Refrigeration: (2-8°C)
Time Period
7 hours
24 hours

Transport Requirements

Refrigerated; Freeze if possible

Causes For Rejection

Non-EDTA plasma specimen; Gross hemolysis; Clotted lavender-top tube

Methodology

Two-site immunoenzymatic (“sandwich”) assay; Chemiluminescent substrate used to measure reaction with luminometer

Collection Instructions If Written

Observe routine precautions for venipuncture; Mix EDTA and whole blood by inverting tube gently several times

Department

Blood

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