Test Panels

Triiodothyronine: Total T3

Overview of Test

This assay is a paramagnetic particle, chemiluminescent immunoassay for the quantitative determination of Triiodothyronine (T3) levels in human serum. T3 is synthesized by the thyroid glad after being stimulated by Thyroid Stimulating Hormone (hTSH). The clinical importance of total serum T3 determination is in the diagnosis of thyroid disorders. Elevated T3 levels can be found in Grave’s disease, and most other classical causes of hyperthyroidism. Decreased T3 levels occur in primary hypothyroid diseases such as Hashimoto’s thyroiditis and neonatal hypothyroidism.

Author

Patrick

Specimen Types

Serum

Test Number

2818

Expected Turn Around Time

24 hours

CPT Codes

84480

Special Instructions

This assay is susceptible to interference from high levels of biotin (>30 mg per day usage).

Preferred Volume

2 mL

Minimum Volume

1 mL

Collection Container or Device

SST

Collection Instructions Attached

No collection instructions attached

Storage Details/ Stability Requirements

Temperature
Room Temp: (15-30°C)
Refrigeration: (2-8°C)
Time Period
8 hours
48 hours

Transport Requirements

Refrigerated

Causes For Rejection

Grossly Hemolyzed, Frozen in Gel Tube, QNS, Not Centrifuged

Methodology

The Total T3 assay is a competitive binding immunoenzymatic assay. A luminometer is used to measure the light generated by the reaction. The light production is inversely proportional to the concentration of Total T3 in the sample.

Collection Instructions If Written

Department

Blood

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