Test Panels

Vitamin D, 25 OH

Overview of Test

Vitamin D is a steroid hormone involved in the active intestinal absorption of calcium and the regulation of homeostasis. In the liver, Vitamin D is hydroxylated at its carbon 25 to form 25-OH Vitamin D. This metabolite is the predominant circulating form of Vitamin D and is considered to be an accurate indicator of the general Vitamin D status of an individual.

Author

Patrick

Specimen Types

Serum

Test Number

4958

Expected Turn Around Time

24 hours

CPT Codes

82306

Special Instructions

Heterophilic antibodies in human serum can react with reagent immunoglobulins or other reagent material, interfering with in vitro immunoassays. Patients routinely exposed to animals, animal serum products, or other immunogenic products that may elicit heterophilic antibody production against the assay’s reagents can be prone to this interference and anomalous values may be obtained.

Preferred Volume

2 mL

Minimum Volume

1 mL

Collection Container or Device

SST

Collection Instructions Attached

No collection instructions attached

Storage Details/ Stability Requirements

Temperature

Room Temp: (15-30 C)

Refrigeration: (2-8 C)

Time Period

48 hrs

7 days

Transport Requirements

Refrigerated

Causes For Rejection

Grossly Hemolyzed, Frozen in Gel Tube, QNS, Not Centrifuged

Methodology

Direct particle-enhanced immuno-turbidimetric assay is used. The assay’s proprietary reagents are designed to dissociate vitamin D from vitamin D binding proteins, found in serum, while particles coated with anti-vitamin D antibodies bind to the dissociated vitamin D, thereby causing agglutination. This agglutination is detected as an absorbance change, with the magnitude of the change being proportional to the quantity of total vitamin D in the sample.

Collection Instructions If Written

Centrifuge and separate serum as soon as possible after collection.

Department

Blood

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25003 Pitkin Rd, Ste F-300 Spring, TX 77386

1250 Stephenson Highway, STE 200, Troy, MI 48083

Test Panels

Vitamin D, 25 OH

Overview of Test

Author

Specimen Types

Test Number

Expected Turn Around Time

CPT Codes

Special Instructions

Preferred Volume

Minimum Volume

Collection Container or Device

Collection Instructions Attached

Storage Details/ Stability Requirements

Temperature

Room Temp: (15-30 C)

Refrigeration: (2-8 C)

Time Period

48 hrs

7 days

Transport Requirements

Causes For Rejection

Methodology

Collection Instructions If Written

Department

Contact Us

Have a question or comment?

info@totallabsolutions.com

+1 (248) 800-6626

+1 (248) 800-6656

Contact Us

Have a question or comment?

1250 Stephenson Highway, STE 200, Troy, MI 48083

info@totallabsolutions.com

+1 (248) 800-6626

+1 (248) 800-6656

Providers

Patients

Company